My highest is 47.3 mph down
Black Dike descent, riding my flat bar heavy-ish commuter, spinning like crazy in 48x11 with my arse hovering just above the saddle.
What I thought from memory was my second highest ever was 46.0 mph descending the A689 down Weardale, on a road bike (Felt Z70) with a gale force wind to my rear. I didn't realise how fast I was going since I'd set my computer to kilometres fro the Audax I was riding and hadn't twigged how close to my record I was so I was dragging the rear brake. A shame because I know that road very well, and it's a good surface with decent sight lines and with that tailwind I could've really flown down there.
Checking my records, it would appear I actually exceeded that on a yacf September 600 in 2011 (46.6mph, no idea where). This feels unlikely, but not, I guess, impossible.
All of those measured with a very accurately calibrated wired computer.
All of these pre-date my GPS/Strava usage. Strava claims I've exceeded these since, but those are all spurious GPS glitches -- there's no way in hell I spun my 70" fixed at 54.6mph anywhere in Essex, and as for 46.7mph on the flat (this being Holland!) on a 67.5" gear - if I'd really done that in 2012, I would have been expecting a call from Dave Brailsford and a first class ticket to London.