Hi Sam.
At that level, assuming SATA drives, you wouldn not hook them all up to a motherboard.
You need a RAID card with a fanout cable.
A proper server would have a SAS or SATA backplane, but we are going to ofar here.
In fact for your purpose I would today not go for a hardware RAID card.
I would go for an HBA card without RAID. Then use ZFS to connect the drives into a storage pool.
You will need an LSI card with an external connector.
e-SATA connectors exist but I am extremely unsure if you can fan out on those connectors.