Use the road bike to build the muscles, cardio and pulmonary.
Use a simple test indoors in a controlled condition to measure.
The indoor controlled test is the simple ‘Step test’ on and off a box.
If it’s the same box and the same steps per minute for the same duration, at the same room temperature and you are wearing the same clothes, fitness improvement is noticed when the End of Test heart rate reduces.
The fantastic attribute of the step test is you can calculate Watts from first principles using your body mass, the height of lift and frequency of lift.
Then there is a formula using Watts and HR to calculate VO2 uptake.
You can, if so inclined, wear a big belt and as you lose body fat, add on weight disks to compensate, to keep your mass constant.